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Although some newly documented maize mutants illustrate a phenotypic and probably functional conservation of mutated genes compared to their orthologs in rice and Arabidopsis, most maize mutants had distinctive phenotypes representing the divergence between monocots and eudicots and between rice and maize during higher plant evolution.
A salient advantage of the auxotrophic double mutant, illustrated here, is its marked attenuation in immunocompromised mice.
Figure 5 illustrates a mutant P with a different protease digestion pattern than wild-type P. Different partial proteolysis patterns show that the mutations affected the structure of the protein, consistent with disrupted binding at the putative contact site.
Hypnomys illustrates a different case.
To further map the regions of the APP C-terminal domain required for sAPP α-induced Akt activation, we transfected two APP deletion mutants (illustrated in Figure 6a) lacking the YENPTY motif (ΔNPTY) or a central sequence motif (ΔPEER) of the APP C-terminal domain into an APP-deficient background.
Our conclusions are supported by phenotypes resulting from discrete tyrosine to phenylalanine mutants, illustrated in Fig. 9. Three tyrosines in the carboxy-terminal of ZnT3 were subject to site directed mutagenesis Y330 (TLTYHVA), Y357 (SRLYSRF) and Y372 (VEQYQPE).
There were no significant changes in hydrogen exchange for Abl T315I in the presence of dasatinib, illustrating a lack of interaction between dasatinib and this mutant.
The pgd3-umu1 insertion site that co-segregates with a seed mutant phenotype illustrates the utility of the UniformMu FST resource for generating hypotheses about gene function.
They illustrate a sad statistic.
The transposon mutant analysis illustrates the importance of the F. novicida Hfq N-terminal domain during 42°C growth (Fig. 3B).
Moreover, double mutant analyses illustrates that CCS52A2 synergistically interacts with SAM organizers.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com