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This deletion mutant has the potential to circumvent the need for splicing.
The ΔpvdDΔpchEF mutant has the double disadvantage of a longer lag phase and an earlier cessation of logarithmic growth.
One last mutant has the blues, and that's Kurt Wagner Kodii Smit-McPhee), who is very camp, very smiley, and very German.
The sGCΔN mutant has the opposite properties: it produces cGMP but does not localize to the leading edge.
This mutant has the same histidine content and the same residues at positions 18 and 94 than the wild-type LTB5.
This approach is similar to the dSLAM [10] technique, claimed to be using a minimum definition of expected fitness (two mutants are independent if the double mutant has the same fitness that the less-fit single mutant).
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One outstanding question about the z3 phenotype is how mutation of a putative citrate transporter produces variegated leaves, although the all cells in the z3 mutant have the same mutant genotype.
This PrPC mutant had the two charged amino acids of the α-cleavage site substituted by hydrophobic isoleucines.
For instance, the L56A mutant had the highest PA-binding affinity (KD of 4.4 nM) among the mutants tested, which is close to the affinity of CMG2-R40 (2.4 nM).
The TC10CA mutant had the opposite effect.
Furthermore, the MNN10 mutant had the highest level of extracellular protein secretion among the knockout strains.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com