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Functional analysis of a rice grain incomplete filling 1 (GIF1) mutant has revealed that the cause is a single mutation in OsCIN2 (Wang et al. 2008).
However, detailed structural analysis of the Q212P mutant has revealed that α3 helix exhibits a small rotation along the helical axis compared to the WT protein.
Our first analysis of the sgn3 mutant has revealed an unexpected robustness of Arabidopsis growth towards a complete lack of its main root diffusion barrier.
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Mouse knockouts and a Zebrafish germline Dicer mutant have revealed important functions for microRNAs in the coordination of normal embryonic development [18], [19], but the individual vertebrate microRNAs are in general still enigmatic genetic objects and only a few have been characterized at a functional level.
Besides, Chinese hamster ovary cell lines (EM9 and EM-C11) with XRCC1 mutant have revealed an unusually high frequency of sister chromatid exchange induced by alkylating agents, such cells reverting subsequently to the transfection of human XRCC1 [ 26].
Previous results based on an exhaustive, computational evaluation of all pairs consisting of the wild-type and a single-gene knockout mutant have revealed several pairs of improved growth relative to the corresponding monocultures [ 54].
Phenotypic analysis of these mutants has revealed several distinct types of asymmetry mutants.
Characterization of these mutants has revealed that many also have defects in phytohormone metabolism or response [ 11- 15].
The use of Ca2+-binding deficient CaM mutants has revealed the regulation of a number of proteins by partially liganded CaM.
During the past years, characterization of plant cell wall mutants has revealed dozens of genes involved in cell wall synthesis and modification [ 28, 32– 41].
Studying these mutants has revealed the presence of two major stress-related pathways, i.e., ABA-dependent and ABA-independent gene pathways [ 2].
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