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In summary, these results indicate that the ΔpkaA mutant has reduced glucose uptake and metabolism due to reduced hexokinase activity and, therefore, increased the utilization of intracellular stores such as trehalose, required for maintaining normal glycerol and pyruvate levels, which are generated by gluconeogenesis.
The propensity for wild type hLYS to complex with and be inhibited by anionic biopolymers is due in large part to Coulombic interactions, and as expected the functionally enhanced double mutant has reduced cationic character.
An H4 S1A mutant has reduced sporulation efficiency and generates aberrant spores.
Moreover, an fps1Δ mutant has reduced whole-cell and plasma membrane ergosterol levels compared to wild-type cells [ 50].
The amylose-extender mutant has reduced activity of branching enzyme II (BEIIb), causing alteration in the fine structure of grain amylopectin [ 8].
sopA is used to alter host cell physiology and promote bacterial survival in host tissues [ 56]. sopA mutant has reduced Salmonella-induced polymorphonuclear leukocytes transepithelial migration [ 56].
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The I88A mutant had reduced activity, the N77S mutant had strongly impaired activity, whereas the D87A, L97A and L104A mutants had negligible activity.
Gene regulatory network of rad mutant having reduced ADH1 activity.
Unlike xth31 mutant where the Al content of the whole roots is decreased nearly three folds, our ral1 mutant had reduced Al accumulation only in the root tips.
Sh2 encodes the large subunit of the heterotetrameric starch synthesis enzyme, adenosine diphosphoglucose pyrophosphorylase (AGP; EC2.7.7.27), and its mutant had reduced sensitivity to phosphate, an inhibitor of AGP, giving rise to increased seed weight [28].
Our fadD2 mutant had reduced swimming and swarming motilities and decreased virulence factor expression (proteases, phospholipases, rhamnolipids, and lipases), while the fadD1 mutant and the ΔfadD2D1 double mutant only showed an increased swarming phenotype.
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