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Moreover, the mutant has less basal SA.
My earlier comment that the double mutant has less activity than the WT TERT is still a concern.
Figure 1 figure supplement 4D shows that the sir2∆ sas2∆ double mutant has less H4 K16ac, just like the sas2∆.
We noticed that D6N mutant-reconstituted cells had less GSDMD cleavage and pyroptosis upon LPS plus nigericin treatment, which suggested that either matured caspase-1 participated in the cleavage of GSDMD or the D6N mutant has less activity than WT pro-caspase-1.
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Grains of sug-h mutant have less wrinkled feature, which make it more commercially feasible by easy polishing in processing.
The G236E CFAS mutant had less than 1% of the in vitro activity of the wild type enzyme.
Consistent with the genetic interactions, the swr1Δ rrp6Δ double mutant showed a significant upregulation of a subset of SRTs (n = 45) by 1.5-fold or more (FDR < 0.1), whereas the rtt109Δ rrp6Δ double mutant had less of an effect.
However, in HCT116 cells expressing knock-in mutants of STAT3, a STAT3 phosphorylation mutant that interferes with DNA interactions consistently enhanced the basal levels of BMF, whereas a STAT3 acetylation mutant had less impact on the target gene (Supplementary Figure 9b).
The results clearly showed that EBs purified from infected GFP-Rab14wt overexpressing cells were enriched in fluorescent sphingolipids, whereas EBs harvested from infected cells overexpressing both GFP-Rab14 negative mutants had less associated sphingolipids (Figure 8C).
Importantly, the defects in DSB-stimulated translocation in both double mutants had less than a two-fold effect on plating efficiency subsequent to DSB formation, indicating that our determinations of translocation frequency were minimally affected [43].
The genetic background of jasmonate signaling pathway mutants had less effect on WRKY gene expression.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com