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In contrast, the pmt1D mutant grows very poorly in the presence of sorbitol (Figure 6B), but its growth is unaffected by SDS (Figure 6C).
In contrast, the hupAB double mutant grows very slowly and is highly pleiotropic: a number of cell processes, such as cell division, initiation of DNA replication, transposition, and other biochemical functions, are altered and cause a slow-growth phenotype [9], [10].
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Because ubx2Δ deletion mutant grew very slowly in our strain background, we conditionally controlled the expression of UBX2 with a galactose-inducible promoter that can be suppressed with glucose.
The csk1Δ rhp57Δ double mutants grew very slowly (Fig. 3C and data not shown), suggesting compromised genomic stability even in unchallenged cells.
We found that ess1 H164R swi4 Δ double mutants grew very slowly (synthetic sick) at the semipermissive temperature (34°).
As the latter's nrdDG genes lie on the pHG1 plasmid, a pHG1-free mutant strain grows very poorly on nitrate and does not achieve full denitrification.
The seventh mutant strain, cik1Δ, grows very poorly and was not characterized further.
At the non-permissive temperature, sec9-4 mutant cells grew very poorly and failed to form individual colonies, while sec9-4 vmutantouble mutant cells grew and formed colonies.
For the fabT deletion mutant though, no final conclusion regarding its sensitivity to carolacton treatment could be drawn since the mutant strain grew very poorly under the tested conditions and formed very thin biofilms.
However, we found that the quadruple sap mutant cells grow very poorly in sucrose media.
Initially, the mutant cells grew very slowly, were abnormal in shape and likely to burst open.
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