Methods to study mutant genotypes that lead to mutant phenotypes are discussed, particularly the use of model organisms and mutant proteins to study protein interactions that may be important for development of disease.
There is also a need for more considered choice of 'wild type' and mutant genotypes, paying particular attention to genotypes that commonly arise during chronic infection.
The phenotypes considered differ in timing and detail, but this paper and that of Gao et al are linked by revelations clarifying ABP1 mutant genotypes.
Each of the mutant genotypes is stable on vegetative transfer and retains its characteristics without recombination in hybrids, thus establishing each mutant as a genuine allele5.
The model was validated by reconstructing the phenotypic properties of several known mutant genotypes.
Fermentative, photosynthetic, and in vitro hydrogenase activities were assayed in each of the mutant genotypes.
Future studies should standardize key variables associated with DHPS mutant infection as well as examine DHPS mutant subtypes (pure mutant vs. mixed infections) - perhaps even individual DHPS mutant genotypes - so that data can be pooled to better address this issue.
Confirmation of Atpht1 mutant genotypes.
Mutant genotypes are associated with complicated phenotypes of the disease.
Mutant genotypes have only been detected within mixed infections.
