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I am not some misunderstood mutant from a comic book or a horror movie, although some have treated me as such.
A cell-free protein synthesis-based screening platform was used to identify an initial mutant from a randomly mutated CpI library.
This greatly enhanced the recovery of the mutant from a gel filtration matrix, but had little effect on its ability to refold reversibly after heat denaturation.
The utility of this approach is highlighted by the automated determination of a lysozyme mutant from a molecular replacement solution that is in congruence with a model prepared independently by crystallographers.
Furthermore, an SLO deficient mutant from a STSS isolate NIH230 (NIH230slostlosthehe killing activity for PMN (Figure 3A), thereby, strongly suggesting that SLO is a key element for PMN killing mediated by invasive GAS.
In previous work, our laboratory constructed a phoP mutant from a clinical isolate of Mtb by introducing a marked disruption in the phoP gene, generating the SO2 strain which exhibited impaired multiplication in vitro in mouse bone marrow-derived macrophages, and in vivo in a mouse infection model [12].
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In this work, we isolated a dominant mutant from an Arabidopsis activation-tagging mutant pool.
We identified a small grain (sg2) mutant from an EMS mutant library generated from an indica variety Shuhui498 (R498), which is an excellent restorer of hybrid rice.
We have found a soil-surface rooting mutant from an M2 population that was regenerated from seed calli of a japonica rice cultivar, Nipponbare.
We previously isolated a dominant spl-D mutant from an Arabidopsis activation tagged mutant collection and found that SPL controls lateral organ morphogenesis by regulating auxin homeostasis.
Combining the proline mutations with rationally designed mutants from a previous study led to 2400-fold increase in the affinity of the A6 T-cell receptor for Tax-HLAA2.
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