At 42°C, however, the mutant frequency increases to around 80% over a two-hour experiment.
The mutant frequency significatively increased during the fourth week after the exposure: 13.5 [time] 10 -5) in the exposed group versus 6. 9 10(-5) in the control group.
The BIR frequency was reduced by 1.9-fold in the pif1-m2 mutant (P = 0.0076), whereas the BIR frequency increased by 2.4-fold in the mph1 Δ background (P = 0.0002).
There was a strong correlation between the increase in Pfdhfr-ts triple mutant frequency and the increasing SP drug pressure during that period (R2 = 0.9, with a linear model).
For linked gene pairs, formation of double mutants depends on meiotic recombination, whose frequency increases with physical distance at a rate that is specific to a given genomic region.
Similarly, at 7 months of age, the hypothalamus showed a mean mutant frequency of 8.1×10−5 increasing to 14.6×10−5 in 30-month old animals (p = 0.0032).
In addition, other somatic mutations outside the 50%-frequency peak displayed a hyperbolic decay in counts with mutant allele frequency increases, which is a characteristic of cell population expansion with somatic mutation accumulations under a neutral model— mathematically similar to a population genetics model proposed at the individual human level [ 16].
MMS exposure strongly increases mutant frequency in β-pol null cells, but not in wild-type cells.
Despite a small average increase in mutant frequency for smokers, an increased proportion of base substitutions at AT base pairs in smokers (p = 0.2) hinted at a smoking-associated shift in the mutation spectrum.
As the atm-1 mutant has increased frequency of apparent telomere to telomere chromosome fusions, which can result in a variable high frequency of males (Jones et al. 2012), we examined whether the loss of CKU-80 was exacerbating this phenotype.
