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Following a 30-day fixation period, the experiment was terminated and lung DNA was extracted for mutant frequency and adduct determinations.
Schloss-Silverman et al. measured mutant frequency and plasmid nicking in E. coli and serovar Typhimurium recovered from J774 macrophage line.
There was a strong correlation between the increase in Pfdhfr-ts triple mutant frequency and the increasing SP drug pressure during that period (R2 = 0.9, with a linear model).
Genetic distance analysis (phenetic approach) of the QS obtained before the patients started HAART and after more than one year of treatment showed that mutant frequency and mean genetic distance both significantly increased in immunogical responders, and there was a significant increase in both synonymous and non-synonymous substitution rates at the level of HVR1.
Pearson correlation analysis revealed a strong correlation between mutant frequency and DNA adducts (r > 0.964, P < 0.05).
The differences in the HPRT mutant frequency and cloning efficiency between the two study groups were analyzed by the Student t-test.
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Busuttil, R. A., Rubio, M., Dolle, M. E., Campisi, J. & Vijg, J. Mutant frequencies and spectra depend on growth state and passage number in cells cultured from transgenic lacZ-plasmid reporter mice.
Livers from DMN-treated mice exhibited a dose-related 2- to 5-fold increase over control mutant frequencies and remained at those levels for 10 through 180 days postexposure.
During the follow-up, the mean mutant frequencies and genetic distances in the E1/E2 region at T0 and T1 increased significantly in group A (p = 0.04, Wilcoxon's test), but were non-significantly different in group B (p≥0.28, Wilcoxon's test) and group C (p = 0.6, Wilcoxon's test) patients.
To evaluate the complementation of the mutator phenotype, mutant frequencies and mutation rates were determined.
Statistical analyses were performed using R programming language to compare mutant frequencies and mutation spectra between control and treated animals [ 25].
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