Finally, functional analysis using mutant chicken keratin forms based on those found in the human α-keratin mutation database led to abnormal phenotypes.
Finally, we conduct functional analysis using mutant chicken α-keratin forms based on those found in the human α-keratin mutation database.
For RNA expression analyses, RNA was extracted and expression of normal and PE44.1 containing mutant RNA was evaluated by RT-PCR using primers that distinguish the two mRNA forms based on amplicon size (primers DYSF44/45-F DYSF44/45-F45-R [Tande S3]).
One example of this approach has been described in an earlier section, where five organophosphates could be monitored with an array of biosensors based on mutant forms of acetylcholinesterase from D. melanogaster.
To explore their role in intermediate stabilization in more detail, we modeled two single-mutant forms of StEH1 based on the wild-type crystal structure and studied the full reaction profiles for the hydrolysis of the two TSO enantiomers.
The majority of current animal models of AD are based on the overexpression of mutant forms of genes associated with AD linked to aggressive familial mutations [20], [23], [25], [28], [29].
Here, we performed a site-directed screening technique, tethering-fragment based drug discovery, against wild-type and mutant forms of NDH-2 containing engineered active-site cysteines.
Previous work has led to the establishment of an experimental model based on transgenic expression of normal or mutant forms of human TTR in Drosophila flies.
Full cytoplasmic PTP-SL (both the wild type and the C480S mutant) forms complexes with ERK2 which also display higher MWs than similar complexes based on the truncated PTP-SL 212 549 construct.
Based on these experiments, there is strong evidence that mutant forms of BRCA1 cause increased IGF1R activation, leading to a decrease in apoptosis and a concomitant increased survival of malignant cells, which then can proliferate.
