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The resulting set of kinetic data supported that the mutant forms are kinetically unaffected by inhibitors of the first generation, proving their functional resistance to these compounds.
As shown in Figure 11A, wild type cdc25C, the phosphatase inactive C377S, T67A and T130A HA-tagged cdc25C mutant forms are expressed at equal levels after transfection.
Interestingly, when these repeats (as much as a 158-residue central stretch) are deleted from MamJ, mutant forms are still able to complement a M. gryphiswaldense MSR-1 ΔmamJ strain, restoring magnetosome alignment with the MamK cytoskeleton [22].
Barttin localizes to the plasma membrane, whereas mutant forms are retained in the ER [ 29].
Size exclusion chromatography revealed that several mutant forms are less stable than WT MscS as discussed above.
The critical genetic questions concern the specific genes whose mutant forms are involved in creating the DS.
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Mutant forms were selectively bred in China starting some 2,000 years ago.
Notch mutant forms were generated using the Stratagene site-directed mutagenesis kit and primers with appropriately altered amino acid codons.
In certain lines, each of these mutant forms was significantly enhanced relative to the remaining four classes.
Plasmids pQE30-ptsO, -ptsOH30A and -ptsOS61A encoding (His 6-NPr and its two mutant forms, were obtained by amplification of tHis 6-NPrponding andele usits oligonucleotwoes SP65 and SP66, and plasmutantDONR201-ptsO, -ptsOH30A and -ptsOS61A respectively, as templates.
GST-tagged RB and its mutant forms were propagated in BL21DE3 (pLysE) cells.
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