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Moreover, the TGase production of the best mutant for each round presented a fluctuant improving trend.
To investigate the role of other segment polarity genes in the mutual control between these two genes, we have examined wingless and engrailed protein distribution in embryos mutant for each of the segment polarity genes.
A panel of homozygous Arabidopsis plants mutant for each AGO protein was screened for hyper-susceptibility to TCV, a positive strand RNA virus in the genus Carmovirus.
To uncover the roles of specific AGOs in limiting virus accumulation we inoculated turnip crinkle virus (TCV) to Arabidopsis plants that were mutant for each of the ten AGO genes.
To further investigate the antiviral role of AGO proteins we monitored TCV-induced symptoms on a panel of Arabidopsis plants that are mutant for each of the ten AGO proteins and found that an ago2-1 mutant was hyper-susceptible to TCV.
One representative mutant for each identified crtB insertion site is shown.
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ATP consumption assays performed on purified SMARCA4 ATPase protein variants shows differential activity between SMARCA4 WT and K785R and T910M mutants (for each group, n = 3).
A library of insertional mutants for each HA was generated and deep sequenced after passaging to determine where insertions were tolerated in replicating viruses.
We performed genotyping to confirm the T-DNA insertion in the Z3 gene, and obtained homozygous mutants for each allele (Additional file 5: figure S5f).
To elucidate to what extent each enzyme encoded by the SSGs, including SSIIa, is rate-limiting for starch synthesis in the endosperm, we need to develop and analyze a series of IR36 mutants for each SSG.
Regarding the numbers of mutants for each group of operators (columns four to six in the table), we can observe that changes applied to pointcuts (i.e. operators from G1 group) yield the largest number of mutants for all systems except for FactorialOptimiser.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com