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We used rad2Δ mutant for all Rad2C65Δp overexpression related experiments through this study.
(c) Box plot comparing siRNA expression between WT and hrde - 1 mutant for all genes and genes with hrde - 1 -dependent mRNA changes.
In contrast, the IC50 of the I117M + H275Y variant was not substantially different from that of the H275Y mutant for all of the NAIs, further demonstrating the lack of effect of the I117M mutation on NAI sensitivity.
Lal et al. reported that in an Asian breast cancer patient population, individuals who were homozygous mutant for all three common single nucleotide polymorphisms (SNPs) of ABCB1 (C1236T, G2677T/A and C3435T) had significantly higher exposure to the anticancer agent doxorubicin [16].
These combinations included grains produced from plants that were wild-type for all three SBEIIa_A, B and D genes (ABD), double mutant for SBEIIa_B and SBEIIa_D (Abd), double mutant for SBEIIa_A and SBEIIa_D (aBd), double mutant for SBEIIa_A and SBEIIa_B (abD), or mutant for all three genes (abd).
Similarly, a phospho-mimetic mutant for all three casein kinase I sites (101E/127E/378E) did not display significant changes in ubiquitylation activity (Fig. 4E G), indicating that neither mimicking individual nor combined phosphorylation of the casein kinase I sites affect parkin's ubiquitylation activity.
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PIPE mutagenesis utilizing overlapping complementary 5′ primer ends was used to amplify mutant constructs for all mutants.
In Arabidopsis, mutants for all five DELLA paralogs still show basal levels of fertility in the Landsberg erecta (Ler) ecotype, indicating that DELLA activity in Ler is not essential to maintain fertility (Dill and Sun 2001; Fuentes et al. 2012).
Regarding the numbers of mutants for each group of operators (columns four to six in the table), we can observe that changes applied to pointcuts (i.e. operators from G1 group) yield the largest number of mutants for all systems except for FactorialOptimiser.
As the combination of chemical inhibitors for all three MAPK pathways reproduced the LT-induced phenotype in lung epithelial cells, mutants for all six, LT-targeted MKKs were prepared.
To analyze the functional role of PEGs during seed development, we obtained T-DNA insertion mutants for all genes.
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