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Furthermore, the expression of genes with unknown functions is also regulated by Fe deficiency, and a knockout mutant for a mitochondrial Fe-regulated gene shows sensitivity to Fe deficiency compared to wild-type (WT) plants (Ishimaru et al. 2009).
As well, an isogenic mutant for a serum opacity-like factor has been found to be attenuated in pigs [9].
Eye discs predominantly mutant for a gene were obtained using the eyFlp-cell lethal system [26], [41].
Next, we constructed a ybp2Δmad2Δ double mutant in our background (YPH499) to assess the double mutant for a more subtle synthetic fitness defect.
Since the 4E10-resistant Envs from subject-pair 16 also contained a small hydrophobic substitution (G), we examined the SVPC5-KGQI mutant for a similar phenotype.
Indeed, flies mutant for a number of components of the pathway, such as rictor or melted, display very mild growth impairments, but have strong metabolic defects [12], [13], [19].
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We utilized a pair of human diploid fibroblast cell lines isolated from individuals that are either heterozygous or homozygous mutant for an allele of CDKN2A that is linked to familial melanoma.
In this report we describe a zebrafish mutant for MyD88, a central component in TLR and IL1R signaling.
Conversely, reverse genetics takes a defined mutant, for example a knockout mouse, and looks for the functional consequence of this genetic change.
A mutant for ospD1, an antiactivator for the transcription factor MxiE (Parsot et al. 2005), also was more Congo red−positive than M90T-Sm.
Similar phenotypes were observed in mutants for a variety of ATP synthase and vacuolar protein sorting genes generated in a viral insertion-based mutagenesis screen [70], [74].
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