Scanning electron micrographs of eyes generated with the eyFlp/FRT ubiGFP system bearing TOR, PTEN, PTEN over TOR mutant or control clones of animals reared on 10 g/l yeast food.
In cic homozygous mutant eye discs generated in the rbf1 120a heterozygous background, Cyclin E levels are specifically increased at the MF (Fig. 4C).
E2F target genes are consistently expressed at a lower level in cic mutant eye discs generated in the rbf1 120a heterozygous background than rbf1 120a homozygous mutant eye discs (supplementary material Fig. S2).
Scanning electron micrographs of eyes generated with the eyFlp/FRT cell lethal system and almost exclusively composed of PTEN, TOR, PTEN TOR mutant or control tissue of animals reared on 100 g/l and 10 g/l yeast food, respectively.
ept mutant eye clones were generated as described previously [4], [6].
ept,H99 and ept,H99,psn mutant eye clones were generated by crossing either w;;eptX1,Df 3L H99,FRT80B/TM6B or w;;eptX1,Df 3L H99,psn227,FRT80B/TM6B males to yweyFLP P[m-w+;ubiGFP],FRT80B females.
10 Functional Drosophila compound eye generated by fully everted and differentiated eye antennal imaginal disc.
ept mutant eye-antennal tumors were generated by crossing w;;ept2,FRT80B males and yweyFLP P[m-w+]RpL141,FRT80B/TM6B females.
Mutant eye-antennal discs were generated using the eyeless-FLP/cell-lethal system as described (Classen et al., 2009).
Some mutant eyes displayed position-specific defects.
