A VACV mutant expressing a variant of VCP lacking cell surface localisation resulted in an attenuated phenotype similar to that of the VCP deletion mutant after i.n.n
Because the mutant expressed a wide range of Fe deficiency-responsive genes, the OsNAAT1 knockout mutant appears to induce physiological Fe deficiency.
A mutant expressing a truncated SsrA tag was viable in H. pylori, but affected in competence and tolerance to both oxidative and antibiotic stresses.
Furthermore, citrus canker symptoms could be observed in infections of the ΔhrcU mutant expressing a HrcUXAC variant in which the NPTH site has was abolished.
To further confirm that the expression of a truncated P110 does not lead to a MTO phenotype, a M. genitalium mutant expressing a P110 derivative lacking the last 30 amino acids was obtained by homologous recombination (Fig. 8A).
To corroborate this conclusion an H. pylori mutant expressing a derivative of ArsS with a substitution of H94 to alanine was constructed which also exhibited reduced pH-responsive transcription of hp0119 and hp1432 (Fig. 2 and data not shown).
Consistently, previous studies have suggested that actin cables polarization, which is abolished in a bni1Δ bnr1Δ mutant expressing a non polarized activated form of Bni1p, was not required for cell proliferation [38].
Second, a slm1∆slm2∆ double mutant expressing a constitutively-active allele, Ypk1(D242A), is also able to grow.
Recently, a mouse mutant expressing a particular mutation (Myh4 arl) affecting myosin function was characterized.
Importantly, the C. jejuni ciaD mutant expressing a wild-type copy of ciaD produced more severe clinical signs of disease in some mice compared to the ciaD mutant.
At the same time, phosphorylation at the preferential PID target site S1 was neither strongly impaired in d6pk012 mutants when compared to a d6pk012 mutant expressing a complementing D6PK transgene nor clearly induced by auxin.
