We optimize the models using data from wild type and four single receptor mutant experiments and use data from two receptor double mutant experiments in a validation step.
Three genes that were down-regulated (brlA, chiB, tdiB) and two that were up-regulated (ppgA, veA) in the xprGΔ1 mutant (Experiment 4) were analyzed in qRT-PCR experiments using new preparations of RNA (Table 3), and by agarose gel electrophoresis of qRT-PCR products (Supplementary Figure 1).
The different behaviors of PE fluorescence in WT and mutant experiments presented in this work strongly indicate the distinct contributions of B-PE and b-PE in the energy transfer of PBsomes.
The double mutant experiments shown in Figure 5 indicate that the sfp1Δ and the afo1Δ mutations exert their influence on longevity independently of each other.
Inhibitor and mutant experiments were also examined in order to determine the role that the early developmental-related phytohormones, ABA, GA and BR have in regulating the expression of these genes.
We assessed the predictability of the models on the interactions observed in the mutant experiments by comparing the models to the Gold Standard Network.
Taken together with the observations of blind ninaB P315 mutants, experiments performed in the dark, and the Fly Duplex, these results demonstrate that during social learning student flies must be able to visually perceive information from teacher flies and then undergo an active-learning process in order to stably respond by depressing oviposition.
The TCTEX1D4 binding mutants experiment show a decrease in binding but not a complete loss.
To determine if the Rac1 knockdown effects on proliferation and soft agar growth are specific to Rac1, we performed shRNA-resistant Rac1 cDNA mutant rescue experiments in the knockdown cells.
Summarizing the mutant crossing experiments, in lpcat2 × dgat1, oil synthesis, vegetative growth and seed set are severely penalized.
