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Compared with the wild type, the Δldh mutant exhibited increases of 31.0% and 31.4% in cell yield under glucose and xylose cultivation, respectively, probably because knocking out the ldh gene results in increased acetate and ATP levels.
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Compared to the WT cells, the deletion mutant exhibited increased length, with non-constricted septa, reminiscent of the phenotypes exhibited by pneumococcus stkP-KD-TMH mutant cells (Fleurie et al., 2012).
Though not significant, the Δppk2 mutant exhibited increased susceptibility to several antimicrobials compared to WT (Table 1).
However, the hfq mutant exhibited increased crystal violet staining compared to wild-type when grown in MH broth indicating increased accumulation of adherent biomass (Fig. 8).
Consistent with their increased activity in the reporter assay, each mutant exhibited substantially increased nuclear localisation.
Indeed, two mutants exhibited growth defects in keeping with what is known about their luminescence, namely luxO and luxN, luxPQ, cqsS triple mutants (Table 2).
In contrast, the pri2(C434A) single mutant exhibited severe growth defects at 30°C and 37°C.
The glc7-E101Q mutant exhibited slow growth and impaired glycogen accumulation on glucose, but no specific delay in the cell cycle.
This classification is supported by the alteration types seen in the rad27 Δ mutant (described above); only the rad27 Δ mutant exhibited ade2 -min3 tract length increases.
Compared to the wild-type MR-1, the mutant exhibited impaired aerobic growth and a defect in utilizing DMSO in the absence of O2.
Compared with a wild-type control, the I223R mutant exhibited 28- and 12-fold increases in IC50s for oseltamivir and zanamivir, respectively.
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