While GFP-FUS-WT and GFP-FUS-R521H were predominantly localized in the nucleus (Fig. 7c), the FUS-R521C mutant exhibited a few clear cytoplasmic aggregates (Fig. 7c).
Also, P13F mutant exhibited a half-life temperature (T1/2 °C.2 °C higher than 68.2 °C of WT in addition to a 56% greater papain inhibitory activity.
In vitro fibrinolysis assays showed that both the t-PA mutant and the LMWP-attached t-PA mutant exhibited a fibrinolytic potency similar to that of the wild type t-PA.
When the reaction was conducted with citrate as a carbon source, the mutant exhibited a high whole-cell activity of styrene oxide formation (170 U/g dcw) that was similar to the styrene-degradation activity of the wild-type strain.
The mutant exhibited a distinct yellow-green leaf phenotype throughout development.
The best mutant exhibited a threefold activity increase in the conversion of β-phenylalanine compared to the wild-type.
The mutant exhibited a distinct yellow-green leaf phenotype throughout development, reduced chlorophyll level, and arrested chloroplast development.
Targeted Δtri3 knockout mutant exhibited a sharp decline in the production of trichodermin, and trichodermol, which is a substrate for trichodermin production, accumulated.
The most extreme mutant exhibited a severe defect in progression through the cell cycle; on synthetic medium, the cells progressively accumulated nucleus-containing small buds that generally failed to complete bud enlargement and cytokinesis.
In their report, OsNAAT1 knockout mutant exhibited a kind of Fe deficiency symptoms and increased expression of various genes in relation to Fe absorption or accumulation such as OsNAS1, OsIRT1, or OsYSL2.
The R667A SARS-CoVpp mutant exhibited a lack of virus entry enhancement following protease treatment.
