Fig. 5A shows two neighboring hemisegments in a late stage (24 h AEL) prospero17 mutant embryo stained with anti-HRP to visualize motor axon terminals (green), and anti-GluRIIA antibodies (magenta).
The Habenular Neuropil of tcf7l2 Mutants Is Symmetric and Not Devided into Subnuclear Domains, Related to Figure 2 3D reconstruction of a dorsal view with anterior to the top of a 4 dpf tcf7l2 mutant embryo stained for acetylated tubulin (cyan) and SV2 (red).
Wild type sibling (A – E ) and His C mutant (F – J ) embryos stained with DAPI (DNA) and antibodies against Cyclin B (CycB) and β-Galactosidase (lacZ).
Wild type sibling (A – C, G – I ) and His C mutant (D – F ) embryos stained with DAPI (DNA) and antibodies against phosphorylated H2Av (γH2Av).
To evaluate the morphology of a more discrete subset of axonal longitudinal connectives, Df(1 446-20, Ppt1A179T, and Ppt1S77F, and trans-heterozygous Ppt1 mutant embryos were stained with mAb1D4.
To address the question whether p53 affects differentiation in the shh−/− mutant, retinal sections of wild-type, shh−/− and p53−/−shh−/− mutant embryos were stained for markers of retinal cell types.
To test this interpretation, mutant embryos were stained for Caspase-3, a marker of apoptosis (Srinivasan et al., 1998).
Wild type sibling (A, B, E, F ) and His C mutant (C, D, G, H ) embryos stained with DAPI (DNA) and antibodies against phosphorylated H2Av (γH2Av).
To this end we compared the expression patterns of Ubx, Abd-B, en, wg, cad and Dll in wild-type and in Pcl mutant embryos by staining embryos with antibodies against their protein products.
