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ISH to show NMD on the hu2787 fmr1 allele was done in one batch, so that wild-type, heterozygous and homozygous mutant embryos received identical treatments.
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Zyap1 −/− mutant embryos have fewer cells compared to sibling controls.
The rad21-mutant embryos have an accumulation of G2/M cells compared with WT embryos (12).
The embryos receive nourishment from the mothers' uterine fluid.
Thus, the enclosed embryos receive much less UVB.
The ap2 mutant embryos are larger and have increased cell numbers and cell size.
Homozygous His C mutant embryos lack zygotic histone synthesis, but as they derive from heterozygous females they receive maternal histone proteins and mRNAs (Lanzotti et al, 2002).
The mapped mutant with the most unusual phenotype (emb71), described before as external [5] because some mutant embryos at maturity protrude from the base of the seed, was found to be disrupted in YODA, a gene that has received considerable attention in recent years.
This idea received support from recent studies in which maternal folate deficiency was found to increase the rate of cranial NTDs in Pax3 (splotch; Sp 2H ) mutant embryos (Burren et al., 2008; Greene et al., 2009).
(G) Quantification of vacuoles in wild-type and mutant embryos.
We found biallelic mutant embryos and pups, indicating 100% efficiency in base conversion.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com