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(F – H ) His C mutant embryos of comparable age were identified based on the lack of lacZ staining.
Heterozygous mating was used to generate mutant embryos of desired genotypes (determined by PCR reactions) and specific stages described in the text.
Indeed, studying the effects of maternal diabetes on the Wnt-PCP pathway demonstrated that there was strong association with the Daam1 genotype, whereby the embryopathy observed in Daam1 gt/+ mutant embryos of diabetic dams was more severe.
The uneven reduction of CpG methylation between mutant embryos of the same genetic background could lead to erratic de-repression and ectopic activation of caudalising genes within the forebrain, and therefore to a variable phenotype.
Furthermore, we demonstrate that the mutant embryos of abp1-1 and abp1-1s as well as the loss-of-function bsm1-1 allele showed similar embryo phenotypes and are arrested at the globular stage as it was shown in the original studies (Babiychuk et al., 2011; Chen et al., 2001).
To distinguish the autonomous and non-autonomous action of DNase II, we first examined ToLFP signals in the wild-type worm (N2) embryos and two mutant embryos of ced-3 (encoding a homologue of mammalian caspase) and ced-1 (encoding a transmembrane receptor for cell corpse recognition).
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At stages where LPM expression of Pitx2 would be expected, the majority of homozygous mutants had no LPM expression (22/24)(Figure 2L O), but a single mutant embryo of ∼3 somites had expression in the right LPM and another of ∼8 somites had expression in the left LPM in a more restricted domain than wild type (Figure 2K,P).
We compare the endoderm phenotype of aldh1a2i26 and aldh1a2um22 mutant embryos to that of embryos treated with DEAB (a pan-Aldh inhibitor).
While FAK inhibitor had no visible effect on wild-type zebrafish at 5 dpf, it greatly decreased the number and size of epidermal cell masses in S1P2-mutant embryos of the same age.
Surprisingly, we observed no differences between wild-type and zc4h2 mutant embryos for either of these markers (Supplementary material, Fig. S7).
The increased susceptibility of OCRL1 mutant embryos to seizures indicates the early appearance of neurological abnormalities in this model.
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