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Furthermore, while ablation of miR-106b approximately 25 or miR-106a approximately 363 has no obvious phenotypic consequences, compound mutant embryos lacking both miR-106b approximately 25 and miR-17 approximately 92 die at midgestation.
To identify PAL-1 regulatory target genes, we used microarrays to compare transcript abundance in wild-type embryos with mutant embryos lacking a C blastomere and to mutant embryos with extra C blastomeres.
The mutant embryos lacking expression either had hearts that had not yet looped (n = 6) or had ventrally looped hearts (n = 4).
To test the value of DeadEasy software for addressing biological questions we carried out temporal profiles of dying cells, in wild-type and H99 mutant embryos (lacking apoptosis).
In contrast, mutant embryos lacking both maternal and zygotic contributions died during embryogenesis but did not display obvious morphological defects and were indistinguishable from embryos lacking only the cold zygotic contribution (Fig. 4A D).
In zebrafish, mutant embryos lacking mature miRNAs had severe deformity during embryogenesis [ 31- 34].
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Cells in both control and mutant embryos lack positive Caspase 3 signal (Figure 4A1 and 4A2).
Mutant embryos lack expression of isl1 and sst in the endocrine pancreas, but retain isl1 expression in the CNS.
golden mutant embryos lacked melanophores on the head and the trunk at this stage.
Lakritz mutant embryos lack all retinal input as they fail to specify RGCs.
Mutant embryos lack Tie-2 protein, but do not display any defect in heart and vessel development.
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