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Moreover, the overexperssion of dMib1 in dneur mutant Drosophila cannot rescue the cuticular neurogenic phenotype of dneur mutant embryos, indicating a non-redundant function of Neur in this context [10].
mz- fh131 were identical to zygotic fh131 mutant embryos, indicating that its role in neuronal migration is the earliest detectable function for this gene (Fig. 1D,D′).
(C and D ) BrdU was detected in the epidermis of His C mutant embryos indicating DNA replication but the pattern of replicating cells was distinct from wild type.
In addition the expression of these makers was not altered in the mutant embryos indicating that vasa null SGPs maintain appropriate marker gene expression.
All five genes are downregulated in the medial part of the dorsal telencephalon of Ngn2 mutant embryos, indicating that Ngn2 is indeed required for normal expression of these genes).
We found that the mitotic index of the CPCs (Isl1 and PiH3 dual-positive cells) in the mutant embryos (AHF-Cre; Cdh2fl/fl) was significantly lower than that in the control embryos, although the mitotic index in the neuroepithelial cells of brain remained the same for both the wild-type and mutant embryos, indicating that the decrease in proliferation was specific to the Isl+ cells within AHF.
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Our analysis of bou cold double mutant embryos indicates that at least these two genes do not exert redundant functions during SJ assembly and cell polarity establishment.
Taken together the various developmental defects displayed by Sp2lzn/lzn mutant embryos indicate that Sp2 is required for normal embryonic development at late embryonic stages.
The molecular phenotype of the Tbx6 mutant embryos indicates a disruption of nodal signaling and is similar to the phenotype of embryos with decreased levels of Nodal expression around the node [26].
Western blotting of homozygous mutant embryos indicated that protein expression was reduced by approximately 70% in this line, herein referred to as OCRL1−/− (Fig. 3B, top).
Therefore, the decreased volume of apoptotic particles observed in mutant embryos indicates that neurons may disappear through caspase-independent cell death or accelerated glial phagocytosis.
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