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Taken together the various developmental defects displayed by Sp2lzn/lzn mutant embryos indicate that Sp2 is required for normal embryonic development at late embryonic stages.
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Our analysis of bou cold double mutant embryos indicates that at least these two genes do not exert redundant functions during SJ assembly and cell polarity establishment.
The molecular phenotype of the Tbx6 mutant embryos indicates a disruption of nodal signaling and is similar to the phenotype of embryos with decreased levels of Nodal expression around the node [26].
Moreover, the overexperssion of dMib1 in dneur mutant Drosophila cannot rescue the cuticular neurogenic phenotype of dneur mutant embryos, indicating a non-redundant function of Neur in this context [10].
mz- fh131 were identical to zygotic fh131 mutant embryos, indicating that its role in neuronal migration is the earliest detectable function for this gene (Fig. 1D,D′).
Western blotting of homozygous mutant embryos indicated that protein expression was reduced by approximately 70% in this line, herein referred to as OCRL1−/− (Fig. 3B, top).
Therefore, the decreased volume of apoptotic particles observed in mutant embryos indicates that neurons may disappear through caspase-independent cell death or accelerated glial phagocytosis.
(C and D ) BrdU was detected in the epidermis of His C mutant embryos indicating DNA replication but the pattern of replicating cells was distinct from wild type.
In addition the expression of these makers was not altered in the mutant embryos indicating that vasa null SGPs maintain appropriate marker gene expression.
Counting the DAC-positive neurons in H99 mutant embryos indicated a significant increase in their number compared with wild-type (wt) embryos, indicating that these neurons normally die through apoptosis.
All five genes are downregulated in the medial part of the dorsal telencephalon of Ngn2 mutant embryos, indicating that Ngn2 is indeed required for normal expression of these genes).
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