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We further investigate whether epiboly defects in MZ spg mutant embryos devoid of Pou5f1/Oct4 may be caused by changes in intercalation behavior.
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This antibody cannot react with ΔCD98hc-β geo mutant protein (Table 1), thus embryos devoid of M-20 staining should be homozygous mutants (CD98hcΔ/Δ).
For embryos devoid of Pou5f1 function maternal and zygotic spg m793 mutants (MZ spg m793) were used.
(G) Quantification of vacuoles in wild-type and mutant embryos.
We found biallelic mutant embryos and pups, indicating 100% efficiency in base conversion.
In this study, we successfully observed this phenomenon in Rab10 mutant embryos.
The ap2 mutant embryos are larger and have increased cell numbers and cell size.
(C) Quantification of Ki67-positive cells in wild-type and mutant embryos.
Taken together, these data demonstrate that the development of Rab10 −/− embryos is defective approximately at E7.5 and the mutant embryos are totally resorbed at E9.5.
To determine the exact time of death of the homozygous mutant embryos, we isolated and examined embryos generated from the Noc4l+/- intercrosses at different days of gestation.
Mutant embryos were collected from indicated crosses.
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