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Our mutant embryos appeared to survive at least to E7.5.
Consistent with the involvement of Ezh2 in erythropoiesis in the developing liver (Mochizuki-Kashio et al., 2011), Ezh2 mutant embryos appeared anemic (Fig. 1; supplementary material Fig. S3A).
Defects in segmentation became even more apparent at 25% DT, when mutant embryos appeared shorter due to compacted segments, and the En stripes were almost twice as broad as those of WT.
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Interestingly, the blastocysts isolated from E3.5 Pik3c3 mutant embryos appear normal compared with controls and display similar growth and proliferation capability in the first two days in culture.
Electron microscopic analysis revealed that epiblast cells in mutant embryos appear normal, whereas the visceral endoderm cells contain larger vesicles inside the lipid droplets.
Thus, homozygous mutant embryos appear to die between E7.5 and E9.5.
Mutant embryos appear to be normal apart from a distinct cardiac phenotype of a shortened OFT with abnormal looping (Fig. 6C,D).
All mutant embryos appear to survive infection with E. coli; however, injection with M. luteus selectively and significantly reduced the survival of modSP 1 and psh 1 ; modSP 1 double mutant embryos, confirming that the Toll pathway is necessary for the resolution of Gram-positive infections at this stage of Drosophila development.
By contrast, in the Neurl4 mutant embryos CP110 appeared in bright foci which were usually apical to the nuclei (Fig. 5C).
In contrast to defects observed in the CNS in elmo; spg double mutants, analysis of the final muscle pattern in these embryos appeared wild-type (Figure 4B).
At E9.5 Sp2lzn/lzn embryos appeared apparently normal.
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