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Moreover, the ubc4Δ mutant does not have an impaired ability to remove core components in G1.
The mutant does not have the A120L mutation that adds stability to the P3Shift mutant.
The Mre11 nuclease is required during the de novo synthesis of telomeres at a DSB with a telomere seed sequence, though an mre11 nuclease deficient mutant does not have short telomeres [42].
This is the first study demonstrating that the p.Cys134Trp mutant does not have a strong impact on FOXL2 localization, solubility, and transactivation abilities on a panel of proven target promoters, behaving neither as a dominant-negative nor as a loss-of-function mutation.
The lpcat2 mutant does not have a significant oil phenotype compared to its null segregant-i.e. it is similar to WT.
The lack of higher levels of KD protein in the insoluble fraction of the homogenized kidney further suggests that this mutant does not have a greatly increased propensity to aggregate (Supplementary Material, Fig. S9B).
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Except for the pale phenotype of ppi2 (attoc159 mutant), attoc132, attoc120 and ppi4 (attoc90) single mutant did not have any visible phenotype.
Similarly, infection with the OL-deficient mutant did not have a significant effect on fibrin D-dimer generation.
Figure 1B shows that this mutant did not have a DN phenotype, since it was unable to inhibit the toxicity of the wildtype toxin when used a sub-stoichiometric or equimolar ratios.
We found, however, that the sea3 Δ mutant did not have prolonged Rad53 hyper-phosphorylation as compared to wild-type.
For Tgfbr2, luciferase assay also showed ∼50% decrease of GL activity while miR-93 mutant did not have such effect (Supplementary Figure S18a and b).
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