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The yox12A mutant does not display an increase in HU sensitivity.
As expected, this AGS3 mutant does not display the band corresponding to the slowest-migrating species of AGS3 (Figure 5A).
Thus, the oxt6 mutant does not display a general or global alteration in responses to abiotic stress, but rather a more limited change in the susceptibility just to ROS elicited, for example, with MV.
In contrast the F508delCFTR mutant does not display this cleavage pattern.
Furthermore, a NEDD8 mutant harboring the "ubiquitinizing" Ala72Arg mutant does not display any binding in our assay.
Secondly, pfk2 and not pfk1 mutant does not display the characteristic NADH "spike" after glucose addition, an observation which will be the subject of further investigations.
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Interestingly, the F4W mutant did not display any change in its thermal unfolding pattern, which was similar to that of R-BSX (Figure 2A).
Interestingly, the S303/307A double HSF1 mutant did not display enhanced activity over the S303A mutant (Figure 1B) suggesting that phosphorylation of both S303 and S307 modulate HSF1 repression through similar mechanisms.
In contrast, TPC2 and its mutant did not display effects on autophagosome numbers when expressed on their own (Fig. 7A).
This was surprising because the lon-2 ; sdn-1 double mutant did not display significantly enhanced suppression compared with either of the single mutants.
This relocalization was mediated by phosphorylation of Ser, as the PKA-insensitive Ser358Ala mutant did not display any change in localization.
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