Crosses with these mutually non-complementing mutants do indeed confirm their status as dpl mutants, i.e. they produce wild type F1 adults when crossed to dpo, dpv, or dpov mutants and F1s from crosses to dpol, dplv, and most dpolv flies do not survive to adulthood (see Table 1).
This suggests that these mutants do, indeed, have a functional effect, at least in vitro.
Three mutants did indeed bind tetracycline, G44V, R70G and G346V, thus highlighting these as useful candidates for structural studies.
But is auxin-independent ARF signalling relevant to flowering plants, or is ARF function dependent on a functional CTD? CTD-deficient arf mutants do indeed have aberrant phenotypes.
Mutation in the fission yeast FBH1 gene leads to a phenotype and genetic interactions that resemble those of srs2 mutant, suggesting that spFbh1 does indeed act as an anti-recombinase [95 97].
We have now revealed that both Rab3GAP1/ rbg-1 and Rab3GAP1/ rbg-2 mutations can suppress the RAB-3 mis-localization phenotype in snt-1 mutants, implying that Rab3GAP does indeed function as a complex to participate in the SNT-1-mediated regulation of the RAB-3 cycle.
When the activity of this mutant was tested in the oxygen electrode assay, we now obtained even faster kinetics than the C85/131A mutant, suggesting that a disulphide does indeed exist between cys99 and cys104, which can also modulate Ero1α activity.
Ago251B, dfmr13 double mutant larvae did indeed display a dfmr1 null-like phenotype with respect to the gross morphology of the NMJ.
Thus Fbxl7 mutant cells do indeed behave as supercompetitors similar to loss-of-function mutations in ft or in core components of the Hippo pathway such as hpo or wts (Tyler et al., 2007).
Western blot analysis of cell extracts from the Rpn12 mutant strain did indeed show an accumulation of ubiquitylated proteins, suggesting impaired proteasomal function presumably as a consequence of the reduced incorporation of Rpn10 in the proteasome [ 23].
