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Among all mutants, Ala258Phe mutant displayed the highest activity of 7.59 U mg−1 and nearly 8-folds higher kcat/Km value towards xylose than wild-type BsGDH.
In addition, the T130M/E133F double mutant displayed the largest shift in thermostability with 3.3-fold improvement of t1/2 at 40 °C and a 5.0 °C higher T1/210 min.
The atips1-2 mutant displayed the same phenotype (Figure 2B).
The C600 hupAB mutant displayed the characteristic small-colony and cell-filamentation phenotypes, as expected and observed previously [9].
In support of this assumption, the smf-3 ok1035) smf-3 ok1035ayed the highest resistance to mutantosure with a Ldisplayed mM (Fig. 4).
We speculate that the spb1DA/snr52Δ double mutant displayed the most severe phenotypic defects because loss of methyl groups on 2 adjacent bases had the greatest effects on local hydration spheres, thus producing the most dramatic changes in rRNA structure.
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However, the patterns of association with CNX/CRT are variable, with the Δ7 terminally misfolded mutant displaying the longest retention time.
Strikingly, we show that the catalytically inactive C/S mutant displays the same level of PTP activity as the wild type protein.
This is also consistent with the rfa2 -Dmutantmutant displaying the most severe damage-sensitive phenotype.
The per ; tim double mutant displays the per phenotype, and the cyc ; per double mutant displays the cyc phenotype, as would be predicted from the circadian clock transcriptional feedback loop, which undergoes circadian rhythms in this tissue.
In contrast, E650 mutants were generally insensitive to strontium activation, and the mutant displaying the highest sensitivity was again E650R, in keeping with our findings regarding calcium activation.
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