Interestingly, fadD mutants displayed alterations in swimming and swarming motility and the production of lipases, phospholipases, rhamnolipids, and proteases.
This conclusion is consistent with previous work which showed that RVE8 binds the evening element both in vitro and in planta, and that rve8 mutants display alterations to their circadian rhythms (Rawat et al., 2011; Fariñas and Mas, 2011).
Several additional mutants that displayed alterations in peroxisome size and/or number were identified (see the Yeast Peroxisome Cellular Imaging Resource at http://pbeid.systemsbiology.net).net
TAF4 mutant livers display alterations in parenchyma and lobule organisation with disorganised hepatocyte disposition in lobules and non-aligned hepatic plates due to variations in cell size.
These act7;aip1-2 double mutants displayed striking alterations of shoot architecture, such as twisted stems and siliques abnormally oriented along the shoot, when compared with the single mutants (Fig. 3D-G; supplementary material Fig. S3B-F).
The triple mutant displayed a dwarf phenotype more severe than that of the extreme GA-deficient mutant ga1-3.
The atips1-2 mutant displayed the same phenotype (Figure 2B).
The ΔF4 mutant displayed less enzyme activity than R-BSX.
RNA-seq data showed that both mutants display similar alterations in the expression of genes related to: transcription, mRNA processing, mRNA splicing, translation, light signaling, response to hormones and both abiotic and biotic stress.
Notably, with exception of the brown midrib phenotype, the bmr mutants display little alteration in gross morphogenesis compared with WT, but as a feedstock have lower lignin content and thus greater cellulose digestibility [ 23, 27].
The CYP709B T-DNA insertion mutants displayed no visible phenotypic alterations under normal growth conditions.
