Although tph-1 mutants did have decreased daf-7 mRNA, mod-5 mutants and exogenous 5-HT treatment did not (Supplementary Fig. 3b).
Our results show that erg6Δ mutants do have a longer generation time in liquid medium.
Seven day-old exocyst mutants do have fewer layers of differentiated starch-stained columella cells compared to Col-0 (consistent with the overall observation of shorter developmental zones in the root meristem; Additional file 1: Figure S8-I), but unlike the icr1 mutant the starch staining pattern persists in the mutants after two weeks of growth (data not shown).
Like folM, a folX deletion did not change the MIC in trimethoprim, but the mutant strain did have a competitive advantage over the wild-type strain in trimethoprim (Figure 2).
Deletion of one gene (orf19.4658) caused a twofold decrease in MIC, whereas the other 22 mutants tested did have no significant effect on MIC (data not shown).
Additionally, although GDF5 mutant mice do have fusion of the tarsal bones, they do not display the marked medial deviation of the hindlimb phalanges seen in Tak1col2 mice, indicating that this deviation is not simply secondary to ankle bone malformation.
Here, we ask whether orientational ordering of helix-forming segments occurs early on during folding of a downhill λ6 85 mutant, and how much it correlates with the thermodynamics and kinetics of various λ6 85 mutants that do have folding barriers.
Taken together our WT1 knockdown experiments show for the first time that mutant WT1 proteins do have a biological function and this is linked to gene regulation in Wilms tumour cells.
Curiously, while the single Q1235A substitution had a significant effect on the rate of reaction, the triple mutant did not have any further defect in catalysis than the double R1239/H1242 mutant.
Except for the pale phenotype of ppi2 (attoc159 mutant), attoc132, attoc120 and ppi4 (attoc90) single mutant did not have any visible phenotype.
