Sentence examples for mutant detection was from inspiring English sources

Mutant detection was carried out by Cel1 digestion followed by analysis on a capillary ABI3730 sequencer (Applied Biosystems, Foster City, California, USA) as described in [ 14].

Mutant detection is conducted by extracting DNA from the tissues of exposed lacZ-carrying transgenic rodents, packaging of the lacZ transgene into bacteriophage and infecting host E. coli.

The cycling profile of the PIK3CA mutant detection assay was as follows: incubation at 95°C for 10 min followed by 40 cycles of 94°C for 30 s and 51°C for 1 min. Analysis of the PCR data were performed using QuantaSoft software (Bio‐Rad laboratories, Hercules, CA, USA).

As low as 0.86 fM mutant DNA was detected by this assay, and positive mutation detection was achieved with a wild-type to mutant ratio of 10,000 1.

PAC also identified exon-skipping mutants in clinical cancer specimens although detection was compromised due to heterogeneous (wild-type) transcript expression.

An activity assay together with CD analysis and Western-blot detection was used to evaluate mutants.

The established lower limit of detection was 4 cfu of mutants per lung.

Reversed forks detection was reduced in sac3 radoubleuble mutants.

The cut-off value defined for reliable mutation detection was set as a frequency of 5% mutant alleles.

Globally, the sensitivity of detection was strongly improved by combining growth conditions and by including mutants of transcriptional regulators.

Limit of detection was calculated by Probit analysis at 95% detection level as 3% of mutant allele burden.