Sentence examples for mutant detection is from inspiring English sources
Mutant detection is conducted by extracting DNA from the tissues of exposed lacZ-carrying transgenic rodents, packaging of the lacZ transgene into bacteriophage and infecting host E. coli.
Mutant detection was carried out by Cel1 digestion followed by analysis on a capillary ABI3730 sequencer (Applied Biosystems, Foster City, California, USA) as described in [ 14].
We then relate the net growth of mutant cells to the net growth of sensitive cells via the relative fitness (see Supplement S1); the mean mutant population at the time of detection is the sum of the descendants of all of the mutants that arose during the growth of the sensitive population.
The cycling profile of the PIK3CA mutant detection assay was as follows: incubation at 95°C for 10 min followed by 40 cycles of 94°C for 30 s and 51°C for 1 min. Analysis of the PCR data were performed using QuantaSoft software (Bio‐Rad laboratories, Hercules, CA, USA).
This, together with the fact that the sensitive cell population at the time of detection is large enough that many different mutants have arisen, means that the mutant numbers at the time of detection are approximated by an α-stable distribution.
The positive mutation detection is achieved with a wild-type to mutant ratio of 5000 1.
KDEL detection is normal in epidermal cells of hau and gho mutant stage 16 embryos.
Earlier detection is critical.
Early detection is crucial.
