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A Tos17 insertion line of the APIP12 gene in ZH11, TosRs_04Z11BB04, is obtained from the Rice Mutant Database (http://rmd.ncpgr.cn/).ncpgr.cn/
The NA indicates that this gene has no mRNA record in GenBank.eThe phenotypes of the inserted mutants of OsOPTs except of OsOPT9 were from different mutant database (OsYSL2, OsYSL12, and OsOPT3 from Rice Tos17 Insertion Mutant Database: http://tos.nias.affrc.go.jp/index.html.en, OsOPT4 from Oryza Tag Line: http://urgi.versailles.inra.fr/OryzaTagLine/cgi-bin/focus_mutant.pl).
Phenotypic information derived from both the forward screen and no-phenotype plants was deposited at the updated mutant database: http://www.soybase.org/mutants.org/mutants
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Then, two mutant databases (OTL: http://urgi.versailles.inra.fr/OryzaTagLine/cgi-bin/focus_mutant.pl and NIAS: http://tos.nias.affrc.go.jp/index.html.en) were further searched using the plant names of the inserted mutants in Additional file 1: Table S1 to obtain the observed phenotypes of these mutants.
Development and maintenance of databases is a costly activity and while it is hard to generalize about average costs as these will vary depending on the resource's size and complexity, personnel costs in different countries, etc., we can give an example for the European Mouse Mutant Archive (EMMA) database (http://www.emmanet.org/).org/
The whole panel of 104 p53 germline missense mutants from the IARC R11 database (http://p53.iarc.fr) cloned in the pLS-Ad vector (providing for a constitutive expression of p53 mutants) [ 42] were used to test transactivation capability towards the miR-34a p53 RE.
By searching the SALK Institute sequence-indexed insertion mutant collection (http://signal.salk.edu/cgi-bin/tdnaexpress) and the GABI-KAT database (http://www.gabi-kat.de/), we identified one T-DNA insertion mutant of the GCL2 gene, GABI_115D02.
Both cell lines have been reported to express only mutant p53 and not wild-type p53 (IARC p53 database (http://www.iarc.fr/p53/index.html)), and we confirmed this finding by direct cDNA sequencing, which showed no wild-type p53 (data not shown).
4,033 independent mutant families were phenotyped and the results organised in the UTILLdb database (http://urgv.evry.inra.fr/UTILLdb).inra.fr/UTILLdb
The growth phenotypes of each mutant are summarized in Table 1 and also described at the Candida CGD database (http://www.candidagenome.org).org
To date, 305 different mutations are listed in the ARPKD/PKHD1 Mutation Database (http://www.humgen.rwth-aachen.de1), accounting for more than 700 mutant alleles.
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