Although no mutant data are available for C. cinereus ctp1, BLAST searches identify a putative ortholog containing a CtIP C-terminus domain (data not shown) (Marchler-Bauer et al. 2013).
In the mutant data there is one point for V*, zero for C*, and one for A* that could be significant outliers, out of 41, 49, and 27 observations, respectively.
Similar results were obtained with the mAb Wh-2-11-1, where the fluorescence intensity increased 3.5 fold for the wild-type ADANTS13, but only 1.9 fold for the mutant (data not given).
Similar results were observed for the sec1.1 mutant (data not shown).
No obvious mutant phenotype, however, was observed for the sofl2-1 mutant (data not shown), suggesting that there may be functional redundancy between AtSOFL1 and AtSOFL2.
No difference was observed in the SS activity pattern between the Atss2-1 line and wild type, and the wild type pattern was also observed for the Atss2-2 mutant (data not shown).
When only data from the Sln1 branch active deletion mutant (Sln1 data) is used for parameter estimation, validation using the Sho1 branch active deletion mutant data (Sho1 data) can be very misleading.
Because the affinity of LOPC for SBL1 might vary among the R1 mutants, kinetic data for representative mutants were obtained (Table S1 of the Supporting Information).
Yet, to date, few algorithms have been proposed for systematically using genome-wide mutant fitness data for gene regulatory network inference.
Sample spectra and traces for the nitrite reduction reactions of wild-type neuroglobin and mutants, spectral data for the redox potential determinations of wild-type neuroglobin and mutants, autoxidation rates for WT Ngb, F28W, and H64A at several oxygen concentrations, and a compilation of autoxidation rates, nitrite reduction rates, and redox potential values determined for myoglobin mutants.
