The resulting mutant (T187C, containing two 187 187 disulfide bridges in the tetramer) showed a 200-fold increase in half-life at 75 °C and an increase of 15 deg.
Cyanogen bromide (CNBr) and enzymatic footprints of the photo-labelled receptor and an engineered receptor mutant (L113M), containing an additional CNBR cleavage site, allowed the photoreactive region to be identified as ORL1[107 113] at the C-terminal of TM helix II.
In this study we present a structural and functional comparison between MNEI and a sweeter mutant Y65R, containing an extra positive charge on the protein surface, in conditions mimicking those of typical beverages.
Compared with the empty-vector (pET21a) and predicted protonation-site mutant D407N containing strain, the wild type AcrB constructed in pET21a showed apparent resistance against ciprofloxacin.
The results of replicase assays performed with mutant VP2 containing a deletion in its RNA-binding domain suggests that the essential role for VP2 in replication is linked to the protein's ability to bind the mRNA template for minus-strand synthesis.
Because the L234K and C243A mutations are both located within β2 that appears distorted in these structures, we also constructed a mutant A3G containing both these mutations.
Female mice with cardiac-specific overexpression of mutant HSPB5 containing the R120G mutation, driven by the α-myosin heavy chain promoter, have been described previously [8].
Spontaneous mutant FUS1mut containing Val33Met amino acid substitution was isolated from KRC/Y cell line transformed with wild type FUS1 (unpublished data).
Male mice with cardiac-specific overexpression of wild-type HSPB5 (WT TG) or mutant HSPB5 containing the R120G mutation (R120G TG), driven by the α-myosin heavy chain promoter, have been described [3].
Especially interesting, mutant TLS13, containing an insertion of a VFKQN pentapeptide after Asn60, produced 30-fold higher CAT activity than strain TNP while growing on glucose, and modestly higher expression in fructose and mannose.
