In principle the LOH seen in vfl2 mutants could occur either by increased recombination or increased chromosome loss (Fig. 2).
If these are both equally accessible, one might imagine that binding to mutants could occur in at least conformation A, and so the loss in affinity would be minor (kbTln2).
The hematopoietic tissues (fetal liver, bone marrow, spleen, and thymus) were selected to evaluate by qRT-PCR whether potential compensation for the loss of Stfa2l1 and Csta in mutant animals could occur through increased mRNA levels of Stfa2 and Stfa3 (expressed at significant levels in these organs) as well as Stfa1 (highly expressed in wild-type fetal liver cells) (Figures 2B, S1, and 4A D).
It is therefore interesting to speculate that owing to the altered biomechanical properties of the mutant tendons, some microdamage could occur in the mutant tendon, and the increased number of fused fibrils may be indicative of repair mechanisms in the mutant tissue, which could in turn affect the biomechanical properties of the tissue.
Arguably, p53-dependent regulation of p21waf1 expression in the TP53 mutant cases studies here could occur in leukaemic cells without TP53 loss (14%, 17% and 43%, respectively), but we have no evidence that the increase in total p53 following Tnv-6 treatment associates with enhanced p53-pathway function, making the increase in p21waf1 a likely p53-independent effect.
However, it is unknown if etioplast development is normal in sco1-1 mutantso so the defect could occur before or during seed maturation.
However, indirect regulations could occur in the null mutant of sRNA.
Isolating such mutants is important because continuous TAG degradation could occur and limit oil build up in Chlamydomonas as was shown in plant leaves [ 37].
We also examined whether target gene restoration (TGR) could occur in either type of mutant.
RT-PCR amplification of subgenomic mRNAs was then carried out to check if subgenomic mRNA synthesis could occur in cells transfected with the mutant transcripts at 24 and 72 hours post-electroporation.
