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In the same setting, the mutant could also suppress CDKN1A and several other targets of SLC45A3-ELK4.
Due to the long, thin architecture of sensory dendrites, it is possible that the formation of the Cofilin-decorated actin puncta in the captK304 mutant could also hinder transport within these dendrites.
As expected, the JAK2 F595W/V617F mutant could also support autonomous growth of sorted Ba/F3-EpoR cells to a level identical to V617F (Figure 3A), pointing to an aromaticity requirement at position 595 playing a role in the constitutive activity of V617F.
Strikingly, the triple mutant could also be rescued by Suc feeding.
The Xist A-repeat mutant could also induce histone modifications and other chromatin changes on the X-chromosome.
The inability to isolate an rpoN null mutant could also be due to the poor expression of these essential TCA cycle enzymes in the absence of RpoN.
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Thus, our experimental results demonstrate that the D1 amino acid substitutions responsible for the radiation tolerance in the random mutants, could also account for the improved resistance under high light exposure of the site-directed mutants.
Alternatively, but not exclusively, defects in melanin production in the cell wall of the A. fumigatus color mutants could also allow more efficient release of fungal proteolytic enzymes, which may also induce excessive immune responses within the host.
Around 4 6ss (E8.5), the mutants could also be identified based on their morphology: measurements of the length of the headfold between the pre-otic sulcus and the anterior limit of the forebrain revealed a significant decrease in ablated mutants compared to wild-type embryos (21% reduction; Fig. S2B).
In another scenario, a shortening of T-DNA insertion due to unequal recombination during the creation of the triple-mutant could also result in enhanced BEN1 transcript levels compared with the original ben1-1 line.
Of course, mutants could also be used, if needed.
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