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Primer sequences that amplify TP53 mutations with a sensitivity greater than I mutant copy in 10 000 wild type are shown in Supplementary Table 1.
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These one-step assays were able to detect the mutations at levels as low as 1.5 mutant copies in a DNA sample.
The number of mutant copies in cases with Parkinson's disease tends to be higher than in controls without Parkinson's disease.
The number of G12D mutant copies detected in each spike in plasma is shown in Additional file 3: Figure S2b.
The mutations identified here are heterozygous and thus the patients will likely have equal amounts of both WT and mutant copies expressed in neurons.
Virtually no one inherits two defective copies of a BRCA gene.In someone with one mutant copy, however, the other copy may suddenly fail in a previously healthy cell, unleashing cancer.
Male Fabry patients express one mutant copy of the GLA gene in all cells.
Flies carrying one mutant copy of dCTCF showed reduced RNA levels (30 to 40% of that found in wild type).
The mice had a mutant copy of the tumor suppressor gene adenomatous polyposis coli (APC).
Thyagarajan and Bloom sequenced the viruses that had replicated which meant that the mutant copies of the hemagglutinin protein in these viruses still worked and looked to see where in the protein the changes had occurred.
They also embedded in them mutant copies of a gene called Raf-1.
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CEO of Professional Science Editing for Scientists @ prosciediting.com