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Similar XP reactivation or reversal of I-XCI had already been observed in vivo in mutant contexts in which Xist regulation had been impaired [ 26, 35].
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In this study we have addressed the relative importance of the RAS-effectors, RAF and PI3K, in a RAS mutant context in human tumor lines both in vivo and in vitro using a regulatable RNA interference system.
Repression of a PBoL target transgene by a P-lacZ telomeric locus was tested in aubergine mutant contexts (Table 3).
We then use these metrics to generate databases in order to enable the quantitative phenotypic characterisation of various mutants, of interactions between these mutants, and of the extent of rescue by the forced expression of a gene in different mutant contexts.
TSE between P-lacZ transgenes was found previously to be null in aubergine heteroallelic mutant contexts (Josse et al. 2007).
The authors suggest that this "immobilization of the mobile pool" is correlated to these receptors participating in synaptic transmission in the mutant context but a mechanism to explain this is not offered.
Hence, EBNA1 binds DS with a similar efficiency in both the wild-type and in the mutant context.
The Gap1K9,16R mutant was still down-regulated in this mutant context and only a Gap1 mutant with all nine lysines present in NT replaced with arginine turned out to be protected against degradation and stabilized at the plasma membrane [5].
In this mutant context, either neuronal or glial-specific overexpression of lipases can decrease lipid droplets and ameliorate photoreceptor degeneration.
Expression of NvHoxB or NvHoxE in this mutant context leads to a complete or strong rescue of this phenotype, respectively.
In the wild-type ovary, there is no expression of engrailed or hedgehog in follicle cells [44], [45] and we found that no derepression occurs in these cells for either gene in a ph mutant context (data not shown).
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