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This mutant contains a T-DNA insertion in the promoter of the intron-containing single-copy MIR163 gene, and has undetectable levels of pri-miRNA163 in any of the tissues tested (Fig 1B, upper panel), as well as undetectable levels of mature miR163 (Fig 1B, lower panel).
The first mutant contains a disulphide bridge designed to cross-link remote segments of the polypeptide chain.
The pro-2 mutant contains a single nucleotide substitution, corresponding to a single amino acid substitution in the SAW subdomain of the SlDELLA.
These data can explain why the mutant G121C/Basal mutant contains a faster rate of Meta II decay and why it is different from UV-vis spectrum.
One mutant contains a partial deletion of the DI-domain (EΔ95/295), whereas the other mutant has a large N-terminal deletion leaving only its C-terminal DIII-domain.
Although the traP gene was initially reported to activate the agr system via a pathway distinct from the autoinducing peptide (AIP) pathway [33], Adhikari et al showed that the traP mutant contains a stop codon in agrA, the gene encoding the response regulator, which, in turn, explains the lack of agr activity in the traP mutant [22].
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The Lpat471 mutant contains the predicted amidase domain, whereas the Lpat521 mutant contains an additional 50 residues of the second domain.
The rlk902 mutant contains an activation tag T-DNA insertion [23] at the end of the single intron of the RLK902 gene (Figure 1i).
This construct acts as a dominant negative because the Belgian Blue mutant contains an 11 bp deletion that causes a premature stop in the C-terminus, leaving only the inhibitory propeptide to be expressed [22].
The dominant negative A-Fos mutant contains an acidic amphipathic protein sequence appended onto the N-terminus of the Fos leucine zipper, replacing the normal basic region critical for DNA binding [24].
In the process of addressing this question, we found that the original ausA mutant contains an inadvertent mutation in saeS and, therefore, the observed involvement of aureusimines in staphylococcal virulence was due to that mutation in saeS.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com