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Investigation of the transcriptional products indicated that stable mRNA was produced only by the mutant containing the intron in the sense orientation.
It was not, however, absolutely required for inhibiting the TBP-DNA interaction, based on experiments with the Mot1 latch mutant containing the C-terminal ATPase domain.
This drastically improved DHA production from 0.83 g/l (0.06 g DHA/g glucose) in the ΔtpiA strain bearing cghdpA to 5.84 g/l (0.41 g DHA/g glucose) in the ΔtpiAΔmgsA double mutant containing the same gene.
Expression of one NrbI deletion mutant, containing the actin binding domain, dramatically increased the density and length of dendritic spines with synapses.
In addition, the CLIP-170 mutant containing the HDAC6-interacting domain could partially rescue the migration defects, but the mutants lacking the HDAC6-interacting domain were unable to rescue (Fig. 6A and 6B).
Human APP695 cDNA was used as wild-type or as triple mutant, containing the Swedish, London and Austrian mutations (denoted APP.SLA).
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The DNKR mutant contains the following four mutations in the zinc-finger domain: D94A, N99A, K101A, and R102A.
To determine whether the mutant contained the expected gene replacement, colony PCR was performed using F1 and R3 primers.
The delta-PDZ mutant contains the full EphA2 sequence except for the last five amino acids for PDZ domain binding (AA 1 971) and was designated as EphA2FL-GFP.
while the R-CH mutant contains the CARD and the helicase domains (N-terminal 734 aa).
The Lpat471 mutant contains the predicted amidase domain, whereas the Lpat521 mutant contains an additional 50 residues of the second domain.
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