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This mutant contained a single base change in the regulatory region upstream of the riboflavin biosynthetic genes.
(A) Each pri2 Cys-to-Ala mutant contained a C-terminal triple HA epitope and was integrated at the chromosomal PRI2 locus.
The mutant contained a single intron insertion in the chromosome with expected size of 4.2 kbp.
The Δ phx1 mutant contained a much lower amount of TPP, only about 30% of the wild-type level during the stationary phase (80 h; Fig. 1C).
In contrast, the ΔppsA conidia, when incubated in glucose and transferred to CA plus HU, were able to increase nuclei number, suggesting that this mutant contained a defect after S phase, possibly mitosis, which inhibited germination on glucose.
We sequenced the mutant protein-coding sequence for each allele of Ago1, Dicer-1, Drosha, and Pasha, and except for one Ago1 and Pasha allele, each mutant contained a single change in the coding sequence that altered the polypeptide product (Table 1 and Figure S2).
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Mutations at three positions were introduced to the XYNII mutant containing a disulfide bridge (S110C N154C) in the α-helix.
The first mutant contains a disulphide bridge designed to cross-link remote segments of the polypeptide chain.
Here, we present a lipase mutant containing a biochemical switch allowing a controlled opening and closing of the lid independent of the environment.
An hCaM mutant containing a unique cysteine residue at position 124 on the protein was expressed, purified, and chemically modified with the fluorophore monobromobimane (mBBr).
The pro-2 mutant contains a single nucleotide substitution, corresponding to a single amino acid substitution in the SAW subdomain of the SlDELLA.
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