Sentence examples for mutant constructs revealed from inspiring English sources

Preliminary studies by transfection of cell lines with EGFR mutant constructs revealed that in the absence of ligand (EGF), an enhanced tyrosine autophosphorylation can be observed in cells harbouring the plasmid containing EGFR-R776G when compared with wild-type EGFR, providing biochemical evidence of the functional relevance of this mutation.

Characterization of wild-type PNPO activity and the various mutant constructs revealed a pre-steady state period where the rate of PLP synthesis was slower than that seen between 10 20 min. This was true for all constructs investigated and was also apparent when PNPO was overexpressed in Chinese hamster ovary cells previously (unpublished data).

By contrast, all constructs revealed GUS-positive cells in the cpc try mutant background indicating that this regulation event involves the trichome patterning activators.

Analysis of B4GAT1 enzyme activity in lysates from cells stably overexpressing these constructs revealed strongly reduced activity in the cases of all three mutants, of less than 5% compared to activity levels in wild-type control.

Inspection of the constructs revealed one difference between this and the other constructs.

Although MutationTaster (Schwarz et al. 2014) predicts the ATF6 frameshift variant to initiate nonsense-mediated decay, immunolocalization studies in COS-7 cells overexpressing the mutant ATF6 cDNA construct revealed the production of a smaller protein that is predominantly mislocalized to the nucleus (Fig.  3).

Surprisingly, many plants containing an eGFP fusion construct revealed a mutant phenotype.

Cell wall monosaccharide analysis of the irx9 mutant transformed with the pVND7 IRX9 construct revealed a significant 17% reduction in xylose content compared to the wild type, while the irx7 transformants containing the pVND7 IRX7 construct showed significant reductions between 16-23% compared to the wild type.

To reveal the responsible regions of grouper β-defensin promoter activity, series 5' deletion mutant constructs were constructed in pGL3-basic vector as described previously (19), and used to transiently transfect Ctenopharyngodon idellus ovary (CO) cells and epithelioma papulosum cyprini (EPC) cells (44).

Briefly, cells were transfected with parkin or parkin mutant constructs for 36 h.

Each of these mutant constructs was analysed individually.