Sentence examples for mutant constructs expressed from inspiring English sources
Our observations of type X collagen expression show that, among the mutant constructs expressed in transduced cells, the P5L mutant cell line showed the highest level of type X collagen expression; however, all mutant cell lines expressed considerably less col10a1 than did cells overexpressing wild-type ank.
As seen in Figure 3B, the mutant constructs expressed at the proper MEKK1 protein size of 196 kD.
In addition, -1 frameshift translation appears to be independent of what TIS is selected since all mutant constructs expressed an RF1/2 frameshift product but naturally of different size, which were all detected by the anti-PEG10 antibody and even more specifically by the anti-His antibody (Figure 9).
Surface-accessible Flag immunoreactivity for the indicated DOR-derived chimeric mutant constructs expressed relative to wild-type DOR.
Briefly, after sequence confirmation through DNA sequencing, the mutant constructs were expressed in E. coli strain BL21 with an induction of IPTG (0.25 mM) at room temperature (∼25°C) overnight.
As expected, all mutant constructs that expressed replication-competent RNAs gave rise to a P3 polyprotein precursor that displayed higher mobility than wild type (WT) P3, due to the deletion of two VPg copies (Figure 2B and 2C).
To that end we transgenically rescued e81 UNC-18 null mutants with constructs expressing UNC-18 E465K (orthologous to Munc18-1 E466K).
To determine whether substitution R55W in 2C could restore the plaque forming capacity of FMDV mutants with alterations in 3B (FMDV mutants V3, V19 4 and V15 9, Figure 1), the corresponding mutation was introduced in each of the mutant constructs that express one VPg.
To analyze whether the irregular cleavage of the Y937_M972del mutant α/β-subunit precursor was catalyzed by S1P, the mutant construct was expressed in S1P-deficient SRD-12B CHO cells.
We generated constructs expressing mutant seipin proteins and determined their expression and localisation.
Each of these mutant constructs was faithfully expressed in the 293-cell transient expression assay (see Fig. S2).
