Sentence examples for mutant constructs by from inspiring English sources

We created 3 corresponding mutant constructs by mutating the seed regions of the miR-375 binding sites: PsiCHECKUM-mut1 plasmid contains mutated binding site at position 2276 2282, PsiCHECKUM-mut2 plasmid contains mutated binding site at position 2993 2999, and PsiCHECKUM-mut3 plasmid contains mutated binding sites at both positions as mentioned above.

The fungus Mucor circinelloides J used in this study is a mutant constructed by carbon ion-beam irradiation to improve xylose fermentation.

Consistent with the transposon mutants (Fig. 2A), hemolytic activity was lower in a Δstp1 mutant constructed by allelic exchange and higher in the Δstk1 mutant, compared to WT Newman (Fig. 2B).

An A. caulinodans in-frame hyq operon deletion mutant, constructed by "crossover PCR", showed markedly decreased growth rates in diazotrophic cultures; normal growth was restored with added ammonium as expected of an H2-recycling mutant phenotype.

The G418 resistant rad9, rad24, chk1, pds1 and dun1 mutants (Invitrogen) were crossed with the BY4741 tlc1::HIS3 mutant (constructed by PCR-based replacement of the TLC1 sequence) to obtain heterozygous diploids.

TW30 strain is a toxR deletion mutant constructed by marker exchange-eviction mutagenesis [ 40].

The mutant constructed by Bauer showed more red color, indicating a higher concentration of charge-transfer complexation.

One of the LxCxE binding-defective mutants constructed by our lab contains a single substitution mutation of Asn757 (RB-N, N757F), which is sufficient to disrupt the LxCxE-binding pocket [8].

Growth and development are controlled by a range of separate signalling pathways that can be probed at the molecular level by screening libraries of insertional mutants, constructed by restriction enzyme-mediated integration (REMI).

In comparison, a total of only seven mutants constructed by homologous recombination methods have been reported, two for cipA and five for other genes (ldh, pta, hpt, pyrF, and cel48S) [ 4, 18, 49, 50].

CKI-ε (WT and KD mutant) constructs were provided by Dr. Xiaofan Wang (Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina, USA).