Sentence examples for mutant constructions used from inspiring English sources

Bacterial strains and mutant constructions used are listed in Table 1.

We previously described the technique of B. fragilis 638R transposon mutant library construction used in this analysis [ 25].

For mutant construction we used strains 8C-YUNI101 and E134 described earlier [ 7].

(Popular comical construction used ironically).

24 Bacterial strains, plasmids and oligonucleotides used in this study are listed in tables 1 and 2. For bacterial growth, isogenic mutant constructions 26 27 and transcomplementation experiments, see supplemental methods online.

Oligonucleotides used for mutant construction are listed in table S2.

E. coli strains used for mutant construction were grown in LB media.

Overall, 455 knockout mutants corresponding to genes in the model could be phenotyped (see Figure 1). 1 Italic text indicates the changed carbon or nitrogen source with respect to the medium used for mutant construction (succinate and ammonia).

A complete list of bacterial strains employed in this study is provided in Table 4. Salmonella enterica serovar Typhimurium strain SL1344 JVS-00077) is referred to as wild-type strain and was used for mutant construction.

The mutant constructions were confirmed by sequencing (ABI 3100, USA).

GP carried out RNA extraction, cDNA library construction, Northern hybridizations, RACE experiments, qRT-PCR, mutant constructions and mutant characterizations.